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验证数据展示

  • WB analysis of human saliva using 84140-1-RR

    WB analysis of human saliva using 84140-1-RR

    human saliva were subjected to SDS PAGE followed by western blot with 84140-1-RR (Lysozyme antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84140-1-RR

    IHC staining of human tonsillitis using 84140-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84140-1-RR (Lysozyme antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84140-1-RR

    IHC staining of human tonsillitis using 84140-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84140-1-RR (Lysozyme antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84140-1-RR

    Affinity and Kinetic Characterization of 84140-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84140-1-RR against Human Lysozyme were performed. The affinity constant is 133 nM.

Lysozyme Recombinant antibody

Lysozyme Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84140-1-RR

产品说明书

CloneNo. 241054E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

LYZ, LZM, Lysozyme C, EC:3.2.1.17, 241054E1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman saliva
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84140-1-RR targets Lysozyme in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Lysozyme fusion protein Ag6917 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lysozyme (renal amyloidosis)
别名 LYZ, LZM, Lysozyme C, EC:3.2.1.17, 241054E1
计算分子量 17 kDa
观测分子量10 kDa
GenBank蛋白编号BC004147
基因名称 Lysozyme
Gene ID (NCBI) 4069
RRIDAB_3671701
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61626
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

实验方案

Product Specific Protocols
WB protocol for Lysozyme antibody 84140-1-RRDownload protocol
IHC protocol for Lysozyme antibody 84140-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols