• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 65283-1-Ig (same clone as 65283-1-PBS)

    FC experiment of human PBMCs using 65283-1-Ig (same clone as 65283-1-PBS)

    1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were surface stained with CoraLite® Plus 405 Anti-Human CD11b then intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human M-CSF (CL488-65283, Clone:3G12E6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65283-1-Ig. This data was developed using the same antibody clone with 65283-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65283-1-Ig (same clone as 65283-1-PBS)

    FC experiment of human PBMCs using 65283-1-Ig (same clone as 65283-1-PBS)

    1X10^6 LPS and TNF-α then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human M-CSF (CL488-65283, Clone:3G12E6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated then Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human M-CSF (CL488-65283, Clone:3G12E6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65283-1-Ig. This data was developed using the same antibody clone with 65283-1-PBS in a different storage buffer formulation.

Anti-Human M-CSF (3G12E6), PBS Only

M-CSF Monoclonal Antibody for FC (Intra)
Cat No. 65283-1-PBS

产品说明书

CloneNo. 3G12E6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

3G12E6, CSF 1, CSF1, CSF-1, Lanimostim

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

65283-1-Ig

产品信息

65283-1-PBS targets M-CSF in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 M-CSF fusion protein HZ-1192 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 colony stimulating factor 1 (macrophage)
别名 3G12E6, CSF 1, CSF1, CSF-1, Lanimostim
计算分子量 60 kDa
GenBank蛋白编号BC021117
基因名称 M-CSF
Gene ID (NCBI) 1435
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)