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M-cadherin Polyclonal antibody

M-cadherin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29928-1-AP

Print datasheet

Synonyms

Cadherin 14, Cadherin 15, CDH14, CDH15, CDH3, CDHM, M cadherin, MCAD, M-cadherin, MRD3, Muscle cadherin

验证数据展示

  • WB analysis using 29928-1-AP

    WB analysis using 29928-1-AP

    C2C12 cells were subjected to SDS PAGE followed by western blot with 29928-1-AP (M-cadherin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inC2C12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29928-1-AP targets M-cadherin in WB, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Rat
Immunogen M-cadherin fusion protein Ag25763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cadherin 15, type 1, M-cadherin (myotubule)
Synonyms Cadherin 14, Cadherin 15, CDH14, CDH15, CDH3, CDHM, M cadherin, MCAD, M-cadherin, MRD3, Muscle cadherin
Calculated Molecular Weight 89 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC008951
Gene Symbol M-cadherin
Gene ID (NCBI) 1013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55291
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. M-cadherin (muscle cadherin), also known as CDH15 (cadherin-15), is a 124-kDa type I transmembrane glycoprotein that is highly expressed in developing skeletal muscle, satellite cells, and cerebellum (PMID: 9545347; 12052883). It associates with β-catenin and functions as a cell-cell adhesion molecule in a homophilic and specific manner (PMID: 9545347). M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation (PMID: 1840697).

实验方案

Product Specific Protocols
WB protocol for M-cadherin antibody 29928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols