验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, rat liver tissue, HEK-293 cells, HepG2 cells, Raji cells, THP-1 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse ovary tissue, human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy MAD2L1 Ready-To-Use IHC Kit. MAD2L1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10337-1-AP targets MAD2L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MAD2L1 fusion protein Ag0307 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | MAD2 mitotic arrest deficient-like 1 (yeast) |
| 别名 | Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2 like protein 1, MAD2, HSMAD2 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC000356 |
| 基因名称 | MAD2L1 |
| Gene ID (NCBI) | 4085 |
| RRID | AB_2139365 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13257 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| IHC protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| IF protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| IP protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I. | ||
J Cell Biol Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I. | ||
Oncogene KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. | ||
Cancer Res Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis. | ||
