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验证数据展示

  • WB analysis of HEK-293 using 66014-1-Ig

    WB analysis of HEK-293 using 66014-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66014-1-Ig

    WB analysis of HEK-293 using 66014-1-Ig

    WB result of MAD2L1 antibody (66014-1-Ig, 1:2000) with si-control and si-MAD2L1 transfected HEK293 cells.

  • IHC staining of human testis using 66014-1-Ig

    IHC staining of human testis using 66014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with MAD2L1 antibody included
  • IHC staining of human testis using 66014-1-Ig

    IHC staining of human testis using 66014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66014-1-Ig (MAD2L1 Antibody) at dilution of 1:50.

    Ready-to-use IHC kits available with MAD2L1 antibody included
  • Featured Product
  • KD/KO Validated

MAD2L1 Monoclonal antibody

MAD2L1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66014-1-Ig
CloneNo. 2F5F12
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2 like protein 1, MAD2, HSMAD2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HEK-293
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAD2L1 Ready-To-Use IHC Kit. MAD2L1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66014-1-Ig targets MAD2L1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen MAD2L1 fusion protein Ag17512 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name MAD2 mitotic arrest deficient-like 1 (yeast)
Synonyms Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2 like protein 1, MAD2, HSMAD2
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC000356
Gene Symbol MAD2L1
Gene ID (NCBI) 4085
RRIDAB_11232424
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13257
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.

实验方案

Product Specific Protocols
WB protocol for MAD2L1 antibody 66014-1-IgDownload protocol
IHC protocol for MAD2L1 antibody 66014-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FEBS Open Bio

SETDB2 interacts with BUBR1 to induce accurate chromosome segregation independently of its histone methyltransferase activity

Authors - Yanhong Tu
View Article