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MAD2L1BP Polyclonal antibody

MAD2L1BP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15344-1-AP

Print datasheet

Synonyms

p31(comet), MAD2L1-binding protein, KIAA0110, CMT2

验证数据展示

  • WB analysis using 15344-1-AP

    WB analysis using 15344-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15344-1-AP

    WB analysis using 15344-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15344-1-AP (MAD2L1BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 15344-1-AP

    IHC staining of human skin using 15344-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 15344-1-AP

    IHC staining of human skin using 15344-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A431 using 15344-1-AP

    IF Staining of A431 using 15344-1-AP

    Immunofluorescent analysis of A431 cells using 15344-1-AP (MAD2L1BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, K-562 cells, HeLa cells, A549 cells, HepG2 cells
Positive IHC detected inhuman skin tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15344-1-AP targets MAD2L1BP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAD2L1BP fusion protein Ag7550 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MAD2L1 binding protein
Synonyms p31(comet), MAD2L1-binding protein, KIAA0110, CMT2
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC002904
Gene Symbol MAD2L1BP
Gene ID (NCBI) 9587
RRIDAB_10836940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15013
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.

实验方案

Product Specific Protocols
WB protocol for MAD2L1BP antibody 15344-1-APDownload protocol
IHC protocol for MAD2L1BP antibody 15344-1-APDownload protocol
IF protocol for MAD2L1BP antibody 15344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols