验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, MCF-7 cells, MDA-MB-468 cells, MDA-MB-231 cells, Jurkat cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy MAD2L1BP Ready-To-Use IHC Kit. MAD2L1BP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68336-1-Ig targets MAD2L1BP in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | MAD2L1BP fusion protein Ag7653 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | MAD2L1 binding protein |
| 别名 | Caught by MAD2 protein, CMT2, KIAA0110, MAD2L1 binding protein, MAD2L1BP, RP1 261G23.6 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC002904 |
| 基因名称 | MAD2L1BP |
| Gene ID (NCBI) | 9587 |
| RRID | AB_3085060 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15013 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAD2L1BP antibody 68336-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |