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验证数据展示

  • WB analysis using 68336-1-Ig

    WB analysis using 68336-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68336-1-Ig (MAD2L1BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MAD2L1BP Monoclonal antibody

MAD2L1BP Monoclonal Antibody for WB, ELISA
Cat No. 68336-1-Ig

Print datasheet

CloneNo. 2H3H1

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Synonyms

Caught by MAD2 protein, CMT2, KIAA0110, MAD2L1 binding protein, MAD2L1BP, RP1 261G23.6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, MCF-7 cells, MDA-MB-468 cells, MDA-MB-231 cells, Jurkat cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy MAD2L1BP Ready-To-Use IHC Kit. MAD2L1BP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68336-1-Ig targets MAD2L1BP in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen MAD2L1BP fusion protein Ag7653 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name MAD2L1 binding protein
Synonyms Caught by MAD2 protein, CMT2, KIAA0110, MAD2L1 binding protein, MAD2L1BP, RP1 261G23.6
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC002904
Gene Symbol MAD2L1BP
Gene ID (NCBI) 9587
RRIDAB_3085060
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15013
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAD2L1BP, also named as CMT2 and KIAA0110, belongs to the MAD2L1BP family. It may function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. MAD2L1BP interacts with the spindle checkpoint and coordinates cell cycle events in late mitosis. MAD2L1BP may play an active role to remove MAD2 from p55CDC and tether MAD2 for silencing the checkpoint.

实验方案

Product Specific Protocols
WB protocol for MAD2L1BP antibody 68336-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols