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MAD2L2 Polyclonal antibody
MAD2L2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, A375 cells, fetal HEK-293 cells, HeLa cells, human colon tissue, human kidney tissue, K-562 cells, mouse brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human ovary tumor tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12683-1-AP targets MAD2L2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | MAD2L2 fusion protein Ag3373 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | MAD2 mitotic arrest deficient-like 2 (yeast) |
Synonyms | hREV7, MAD2 like protein 2, MAD2B, MAD2L2, REV7, REV7 homolog |
Calculated Molecular Weight | 211 aa, 24 kDa |
Observed Molecular Weight | 24 kDa |
GenBank Accession Number | BC015244 |
Gene Symbol | MAD2L2 |
Gene ID (NCBI) | 10459 |
RRID | AB_2139530 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UI95 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MAD2L2 antibody 12683-1-AP | Download protocol |
IHC protocol for MAD2L2 antibody 12683-1-AP | Download protocol |
IP protocol for MAD2L2 antibody 12683-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Commun MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair.
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Nat Commun SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination. | ||
J Cell Biol Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset.
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Nucleic Acids Res REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ
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Nucleic Acids Res REV1 and polymerase ζ facilitate homologous recombination repair.
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