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  • Featured Product
  • KD/KO Validated

MAEL Polyclonal antibody

MAEL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 26666-1-AP

Print datasheet

Synonyms

MAEL, maelstrom homolog (Drosophila), Protein maelstrom homolog, RP11 102C16.1

验证数据展示

  • WB analysis of rat testis using 26666-1-AP

    WB analysis of rat testis using 26666-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 26666-1-AP (MAEL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 26666-1-AP

    IHC staining of human testis using 26666-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26666-1-AP (MAEL Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 26666-1-AP

    IHC staining of human testis using 26666-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26666-1-AP (MAEL Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inrat testis tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26666-1-AP targets MAEL in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, RIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MAEL fusion protein Ag24524 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name maelstrom homolog (Drosophila)
Synonyms MAEL, maelstrom homolog (Drosophila), Protein maelstrom homolog, RP11 102C16.1
Calculated Molecular Weight 434 aa, 49 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC028595
Gene Symbol MAEL
Gene ID (NCBI) 84944
RRIDAB_2880595
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAEL antibody 26666-1-APDownload protocol
IHC protocol for MAEL antibody 26666-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,RIP

Andrology

The MAEL expression in mitochondria of human spermatozoa and the association with asthenozoospermia

Authors - Yu-Sheng Cheng
  • KD Validated
View Article
mouseWB

J Cell Mol Med

Loss-of-function variant in TDRD6 cause male infertility with severe oligo-astheno-teratozoospermia in human and mice

Authors - Xinying Bi
View Article