验证数据展示
经过测试的应用
Positive WB detected in | rat testis tissue, mouse testis tissue |
Positive IHC detected in | human testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26666-1-AP targets MAEL in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, RIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | MAEL fusion protein Ag24524 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | maelstrom homolog (Drosophila) |
别名 | MAEL, maelstrom homolog (Drosophila), Protein maelstrom homolog, RP11 102C16.1 |
计算分子量 | 434 aa, 49 kDa |
观测分子量 | 46-50 kDa |
GenBank蛋白编号 | BC028595 |
基因名称 | MAEL |
Gene ID (NCBI) | 84944 |
RRID | AB_2880595 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96JY0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for MAEL antibody 26666-1-AP | Download protocol |
IHC protocol for MAEL antibody 26666-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Andrology The MAEL expression in mitochondria of human spermatozoa and the association with asthenozoospermia
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J Cell Mol Med Loss-of-function variant in TDRD6 cause male infertility with severe oligo-astheno-teratozoospermia in human and mice |