验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, A375 cells, HeLa cells, HepG2 cells, K-562 cells |
| Positive IP detected in | A431 cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55013-1-AP targets c-MAF in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) |
| 别名 | MAF, Transcription factor Maf, Proto-oncogene c-Maf, Proto oncogene c Maf, cMAF |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42-52 kDa |
| GenBank蛋白编号 | NM_005360 |
| 基因名称 | MAF |
| Gene ID (NCBI) | 4094 |
| RRID | AB_10863127 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75444 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for c-MAF antibody 55013-1-AP | Download protocol |
| IF protocol for c-MAF antibody 55013-1-AP | Download protocol |
| IP protocol for c-MAF antibody 55013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity RAS P21 Protein Activator 3 (RASA3) Specifically Promotes Pathogenic T Helper 17 Cell Generation by Repressing T-Helper-2-Cell-Biased Programs. | ||
Acta Pharmacol Sin Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis. | ||
Int J Mol Sci Deletion of Cdk5 in Macrophages Ameliorates Anti-Inflammatory Response during Endotoxemia through Induction of C-Maf and Il-10 | ||
Front Immunol Molecular Mechanisms Driving IL-10- Producing B Cells Functions: STAT3 and c-MAF as Underestimated Central Key Regulators? | ||
J Immunol Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells. | ||
Cell Commun Signal Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis. |
