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- KD/KO Validated
MAFF Polyclonal antibody
MAFF Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human lung tissue |
Positive IP detected in | SKOV-3 cells |
Positive IHC detected in | human ovary tumor tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12771-1-AP targets MAFF in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | MAFF fusion protein Ag3460 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian) |
Synonyms | hMafF, MAFF, Transcription factor MafF, U MAF |
Calculated Molecular Weight | 17 aa, 8 kDa |
Observed Molecular Weight | 18-23 kDa |
GenBank Accession Number | BC015037 |
Gene Symbol | MAFF |
Gene ID (NCBI) | 23764 |
RRID | AB_2137677 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9ULX9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MAFF antibody 12771-1-AP | Download protocol |
IHC protocol for MAFF antibody 12771-1-AP | Download protocol |
IP protocol for MAFF antibody 12771-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Transl Med PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. | ||
J Clin Invest circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF. | ||
Autophagy The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3. | ||
Mol Ther Nucleic Acids miR-320a induces pancreatic β cells dysfunction in diabetes by inhibiting MafF | ||
BMC Genomics Targeted insertion of conditional expression cassettes into the mouse genome using the modified i-PITT | ||
Mol Ther Oncolytics RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.
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