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MAFF Polyclonal antibody

MAFF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12771-1-AP

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Synonyms

hMafF, MAFF, Transcription factor MafF, U MAF



经过测试的应用

Positive WB detected inhuman lung tissue
Positive IP detected inSKOV-3 cells
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAFF Ready-To-Use IHC Kit. MAFF primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12771-1-AP targets MAFF in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MAFF fusion protein Ag3460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
Synonyms hMafF, MAFF, Transcription factor MafF, U MAF
Calculated Molecular Weight 17 aa, 8 kDa
Observed Molecular Weight 18-23 kDa
GenBank Accession NumberBC015037
Gene Symbol MAFF
Gene ID (NCBI) 23764
RRIDAB_2137677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULX9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

实验方案

Product Specific Protocols
WB protocol for MAFF antibody 12771-1-APDownload protocol
IHC protocol for MAFF antibody 12771-1-APDownload protocol
IP protocol for MAFF antibody 12771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang
human,mouseWB,IHC,IF

J Clin Invest

circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF.

Authors - Zhenzhen Chen
humanWB

Autophagy

The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3.

Authors - Eutteum Jeong
mouse,ratWB

Mol Ther Nucleic Acids

miR-320a induces pancreatic β cells dysfunction in diabetes by inhibiting MafF

Authors - Hengzhi Du
mouseIF

BMC Genomics

Targeted insertion of conditional expression cassettes into the mouse genome using the modified i-PITT

Authors - Hiromi Miura
humanWB

Mol Ther Oncolytics

RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.

Authors - Shuyuan Shen
  • KD Validated