MAG Polyclonal antibody

MAG Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14386-1-AP

Print datasheet

Synonyms

SIGLEC4A, SIGLEC 4A, S MAG, myelin associated glycoprotein, GMA



经过测试的应用

Positive WB detected inmouse spinal cord tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14386-1-AP targets MAG in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen MAG fusion protein Ag5845 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myelin associated glycoprotein
Synonyms SIGLEC4A, SIGLEC 4A, S MAG, myelin associated glycoprotein, GMA
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC053347
Gene Symbol MAG
Gene ID (NCBI) 4099
RRIDAB_2878051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

实验方案

Product Specific Protocols
WB protocol for MAG antibody 14386-1-APDownload protocol
IHC protocol for MAG antibody 14386-1-APDownload protocol
IF protocol for MAG antibody 14386-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain

A role of the frontotemporal lobar degeneration risk factor TMEM106B in myelination.

Authors - Tuancheng Feng
mouseWB

Acta Neuropathol Commun

TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.

Authors - Tuancheng Feng
mouseIF, WB

J Cereb Blood Flow Metab

Connexin43 promotes angiogenesis through activating the HIF-1α/VEGF signaling pathway under chronic cerebral hypoperfusion.

Authors - Weiwei Yu
mouseWB

Mol Neurobiol

Quercetin Alleviates Demyelination Through Regulating Microglial Phenotype Transformation to Mitigate Neuropsychiatric Symptoms in Mice with Vascular Dementia.

Authors - Zihu Tan
mouseIF

Neurosci Bull

Digoxin Ameliorates Glymphatic Transport and Cognitive Impairment in a Mouse Model of Chronic Cerebral Hypoperfusion

Authors - Jie Cao
ratWB

CNS Neurosci Ther

Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic-ischemic white matter damage.

Authors - Liu Yang