MAG Polyclonal antibody

MAG Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 14386-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (1)

SIGLEC4A, SIGLEC 4A, S MAG, myelin associated glycoprotein, GMA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse spinal cord tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14386-1-AP targets MAG in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 MAG fusion protein Ag5845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myelin associated glycoprotein
别名 SIGLEC4A, SIGLEC 4A, S MAG, myelin associated glycoprotein, GMA
计算分子量 69 kDa
观测分子量 100 kDa
GenBank蛋白编号BC053347
基因名称 MAG
Gene ID (NCBI) 4099
RRIDAB_2878051
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

实验方案

Product Specific Protocols
WB protocol for MAG antibody 14386-1-APDownload protocol
IHC protocol for MAG antibody 14386-1-APDownload protocol
IF protocol for MAG antibody 14386-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain

A role of the frontotemporal lobar degeneration risk factor TMEM106B in myelination.

Authors - Tuancheng Feng
mouseWB

Acta Neuropathol Commun

TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss.

Authors - Tuancheng Feng
mouseIF, WB

J Cereb Blood Flow Metab

Connexin43 promotes angiogenesis through activating the HIF-1α/VEGF signaling pathway under chronic cerebral hypoperfusion.

Authors - Weiwei Yu
mouseWB

CNS Neurosci Ther

The Neuroprotective Mechanisms of PPAR-γ: Inhibition of Microglia-Mediated Neuroinflammation and Oxidative Stress in a Neonatal Mouse Model of Hypoxic-Ischemic White Matter Injury

Authors - Mingchu Fang
WB

Mol Neurobiol

FGF21 Alleviates Hypoxic-Ischemic White Matter Injury in Neonatal Mice by Mediating Inflammation and Oxidative Stress Through PPAR-γ Signaling Pathway

Authors - Mingchu Fang
mouseWB

Mol Neurobiol

Quercetin Alleviates Demyelination Through Regulating Microglial Phenotype Transformation to Mitigate Neuropsychiatric Symptoms in Mice with Vascular Dementia.

Authors - Zihu Tan
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