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验证数据展示

  • WB analysis of A375 using 55105-1-AP

    WB analysis of A375 using 55105-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 55105-1-AP

    IF Staining of A375 using 55105-1-AP

    Immunofluorescent analysis of A375 cells using 55105-1-AP (MAGEA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAGEA1 Polyclonal antibody

MAGEA1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 55105-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Antigen MZ2 E, Cancer/testis antigen 1.1, CT1.1, MAGE 1 antigen, MAGE1, MAGE1A, MAGEA1, Melanoma associated antigen 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, MCF-7 cells
Positive IF/ICC detected inA375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55105-1-AP targets MAGEA1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
别名 Antigen MZ2 E, Cancer/testis antigen 1.1, CT1.1, MAGE 1 antigen, MAGE1, MAGE1A, MAGEA1, Melanoma associated antigen 1
计算分子量 34 kDa
观测分子量 46 kDa
GenBank蛋白编号NM_004988
基因名称 MAGEA1
Gene ID (NCBI) 4100
RRIDAB_10859942
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43355
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEA1, also named as CT1.1, MZ2-E, MAGE1 and MAGE1A, is the first gene reported to encode a CTL-defined human tumor antigen. It may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. MAGEA1 provided direct evidence for the power of the cDNA expression library as an effective strategy to identify genes encoding tumor antigens recognized by HLA-restricted T cells.(PMID:16951217 ) The antibody is specific to MAGEA1.

实验方案

Product Specific Protocols
WB protocol for MAGEA1 antibody 55105-1-APDownload protocol
IF protocol for MAGEA1 antibody 55105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols