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验证数据展示

  • WB analysis of A375 using 15474-1-AP

    WB analysis of A375 using 15474-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15474-1-AP (MAGEA11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 15474-1-AP

    IF Staining of A375 using 15474-1-AP

    Immunofluorescent analysis of A375 cells using 15474-1-AP (MAGEA11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAGEA11 Polyclonal antibody

MAGEA11 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15474-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA and More (1)

别名

Cancer/testis antigen 1.11, CT1.11, MAGE 11, MAGE 11 antigen, MAGE11, MAGEA 11, MAGEA11, melanoma antigen family A, 11, Melanoma associated antigen 11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells
Positive IF/ICC detected inA375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

15474-1-AP targets MAGEA11 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性human
免疫原 MAGEA11 fusion protein Ag7778 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family A, 11
别名 Cancer/testis antigen 1.11, CT1.11, MAGE 11, MAGE 11 antigen, MAGE11, MAGEA 11, MAGEA11, melanoma antigen family A, 11, Melanoma associated antigen 11
计算分子量 48 kDa
观测分子量 48 kDa, 44 kDa
GenBank蛋白编号BC004479
基因名称 MAGEA11
Gene ID (NCBI) 4110
RRIDAB_2137973
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43364
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAGEA11 antibody 15474-1-APDownload protocol
IF protocol for MAGEA11 antibody 15474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Rep

Integrated analysis of single-cell and bulk RNA-sequencing reveals a novel signature based on NK cell marker genes to predict prognosis and immunotherapy response in gastric cancer

Authors - Jian-Rong Sun
View Article