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MAGEA11 Polyclonal antibody

MAGEA11 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15474-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 1.11, CT1.11, MAGE 11, MAGE 11 antigen, MAGE11, MAGEA 11, MAGEA11, melanoma antigen family A, 11, Melanoma associated antigen 11

验证数据展示

  • WB analysis of A375 using 15474-1-AP

    WB analysis of A375 using 15474-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15474-1-AP (MAGEA11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 15474-1-AP

    IF Staining of A375 using 15474-1-AP

    Immunofluorescent analysis of A375 cells using 15474-1-AP (MAGEA11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells
Positive IF/ICC detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

15474-1-AP targets MAGEA11 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen MAGEA11 fusion protein Ag7778 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma antigen family A, 11
Synonyms Cancer/testis antigen 1.11, CT1.11, MAGE 11, MAGE 11 antigen, MAGE11, MAGEA 11, MAGEA11, melanoma antigen family A, 11, Melanoma associated antigen 11
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa, 44 kDa
GenBank Accession NumberBC004479
Gene Symbol MAGEA11
Gene ID (NCBI) 4110
RRIDAB_2137973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43364
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAGEA11 antibody 15474-1-APDownload protocol
IF protocol for MAGEA11 antibody 15474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Rep

Integrated analysis of single-cell and bulk RNA-sequencing reveals a novel signature based on NK cell marker genes to predict prognosis and immunotherapy response in gastric cancer

Authors - Jian-Rong Sun
View Article