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验证数据展示

  • WB analysis using 83480-5-RR

    WB analysis using 83480-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83480-5-RR (MAGEA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 83480-5-RR

    IF Staining of A549 using 83480-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MAGEA3 antibody (83480-5-RR, Clone: 240435G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83480-5-RR

    FC experiment of HeLa using 83480-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAGEA3 Recombinant antibody (83480-5-RR, Clone:240435G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83480-5-RR

    Affinity and Kinetic Characterization of 83480-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83480-5-RR against Human MAGEA3 were performed. The affinity constant is 73.7 pM.

MAGEA3 Recombinant antibody

MAGEA3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83480-5-RR

产品说明书

CloneNo. 240435G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, Jurkat cells, SGC-7901 cells, NCI-H1299 cells
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy MAGEA3 Ready-To-Use IHC Kit. MAGEA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83480-5-RR targets MAGEA3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MAGEA3 fusion protein Ag0265 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melanoma antigen family A, 3
别名 HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D
计算分子量 314 aa, 35 kDa
观测分子量45 kDa
GenBank蛋白编号BC000340
基因名称 MAGEA3
Gene ID (NCBI) 4102
RRIDAB_3671108
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43357
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.

实验方案

Product Specific Protocols
WB protocol for MAGEA3 antibody 83480-5-RRDownload protocol
IF protocol for MAGEA3 antibody 83480-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols