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MAGEA4 Polyclonal antibody

MAGEA4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55109-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen, MAGE X2, MAGE X2 antigen, MAGE4, MAGE4A, MAGE4B, MAGEA4, melanoma antigen family A, 4, Melanoma associated antigen 4

验证数据展示

  • WB analysis of human placenta using 55109-1-AP

    WB analysis of human placenta using 55109-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 55109-1-AP (MAGEA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 55109-1-AP

    IHC staining of human breast cancer using 55109-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55109-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 55109-1-AP

    IHC staining of human breast cancer using 55109-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 55109-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman placenta tissue, A375 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55109-1-AP targets MAGEA4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma antigen family A, 4
Synonyms Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen, MAGE X2, MAGE X2 antigen, MAGE4, MAGE4A, MAGE4B, MAGEA4, melanoma antigen family A, 4, Melanoma associated antigen 4
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberNM_001011548
Gene Symbol MAGEA4
Gene ID (NCBI) 4103
RRIDAB_10858794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43358
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGEA4, also named as MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

实验方案

Product Specific Protocols
WB protocol for MAGEA4 antibody 55109-1-APDownload protocol
IHC protocol for MAGEA4 antibody 55109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols