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MAGEB18 Polyclonal antibody

MAGEB18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17494-1-AP

Print datasheet

Synonyms

MAGE B18 antigen, MAGEB18, melanoma antigen family B, 18

验证数据展示

  • WB analysis using 17494-1-AP

    WB analysis using 17494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17494-1-AP

    IHC staining of human testis using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 17494-1-AP

    IHC staining of human testis using 17494-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of A549 using 17494-1-AP

    IF Staining of A549 using 17494-1-AP

    Immunofluorescent analysis of A549 cells using 17494-1-AP (MAGEB18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, HeLa cells, A549 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17494-1-AP targets MAGEB18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MAGEB18 fusion protein Ag11624 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma antigen family B, 18
Synonyms MAGE B18 antigen, MAGEB18, melanoma antigen family B, 18
Calculated Molecular Weight 343 aa, 39 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC029525
Gene Symbol MAGEB18
Gene ID (NCBI) 286514
RRIDAB_2138404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M61
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAGEB18 antibody 17494-1-APDownload protocol
IHC protocol for MAGEB18 antibody 17494-1-APDownload protocol
IF protocol for MAGEB18 antibody 17494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols