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MAGEB3 Polyclonal antibody

MAGEB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18000-1-AP

Print datasheet

Synonyms

CT3.5, MAGE B3 antigen, MAGEB3, melanoma antigen family B, 3, Melanoma associated antigen B3

验证数据展示

  • WB analysis using 18000-1-AP

    WB analysis using 18000-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18000-1-AP (MAGEB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 18000-1-AP

    IHC staining of mouse testis using 18000-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 18000-1-AP

    IHC staining of mouse testis using 18000-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18000-1-AP targets MAGEB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAGEB3 fusion protein Ag12476 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma antigen family B, 3
Synonyms CT3.5, MAGE B3 antigen, MAGEB3, melanoma antigen family B, 3, Melanoma associated antigen B3
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC074756
Gene Symbol MAGEB3
Gene ID (NCBI) 4114
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15480
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. MAGEB3, also named CT3.5, is a member of the MAGEB gene family.

实验方案

Product Specific Protocols
WB protocol for MAGEB3 antibody 18000-1-APDownload protocol
IHC protocol for MAGEB3 antibody 18000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols