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验证数据展示

  • WB analysis using 18000-1-AP

    WB analysis using 18000-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18000-1-AP (MAGEB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 18000-1-AP

    IHC staining of mouse testis using 18000-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 18000-1-AP

    IHC staining of mouse testis using 18000-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAGEB3 Polyclonal antibody

MAGEB3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18000-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CT3.5, MAGE B3 antigen, MAGEB3, melanoma antigen family B, 3, Melanoma associated antigen B3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18000-1-AP targets MAGEB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAGEB3 fusion protein Ag12476 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family B, 3
别名 CT3.5, MAGE B3 antigen, MAGEB3, melanoma antigen family B, 3, Melanoma associated antigen B3
计算分子量 346 aa, 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC074756
基因名称 MAGEB3
Gene ID (NCBI) 4114
RRIDAB_3085548
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15480
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. MAGEB3, also named CT3.5, is a member of the MAGEB gene family.

实验方案

Product Specific Protocols
WB protocol for MAGEB3 antibody 18000-1-APDownload protocol
IHC protocol for MAGEB3 antibody 18000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols