MAGEC3 Polyclonal antibody
MAGEC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, mouse thymus tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A375 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IP | See 1 publications below |
产品信息
21491-1-AP targets MAGEC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | MAGEC3 fusion protein Ag15819 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | melanoma antigen family C, 3 |
Synonyms | Melanoma-associated antigen C3, MAGE-C3 antigen, Hepatocellular carcinoma-associated antigen 2, HCA2, CT7.2 |
Calculated Molecular Weight | 643 aa, 72 kDa |
Observed Molecular Weight | 70-72 kDa |
GenBank Accession Number | BC103893 |
Gene Symbol | MAGEC3 |
Gene ID (NCBI) | 139081 |
RRID | AB_10734430 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8TD91 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for MAGEC3 antibody 21491-1-AP | Download protocol |
IHC protocol for MAGEC3 antibody 21491-1-AP | Download protocol |
IF protocol for MAGEC3 antibody 21491-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Commun (Lond) MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma. | ||
BMC Cancer Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells. |