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- KD/KO Validated
MAGED1 Polyclonal antibody
MAGED1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HEK-293 cells, mouse placenta tissue |
Positive IP detected in | A549 cells |
Positive IHC detected in | mouse placenta tissue, human skin tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22053-1-AP targets MAGED1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | MAGED1 fusion protein Ag17047 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | melanoma antigen family D, 1 |
Synonyms | DLXIN 1, MAGE D1 antigen, MAGE tumor antigen CCF, MAGED1, melanoma antigen family D, 1, Melanoma associated antigen D1, NRAGE, PP2250 |
Calculated Molecular Weight | 834 aa, 92 kDa |
Observed Molecular Weight | 95-100 kDa |
GenBank Accession Number | BC014070 |
Gene Symbol | MAGED1 |
Gene ID (NCBI) | 9500 |
RRID | AB_11182601 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y5V3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MAGED1 antibody 22053-1-AP | Download protocol |
IHC protocol for MAGED1 antibody 22053-1-AP | Download protocol |
IF protocol for MAGED1 antibody 22053-1-AP | Download protocol |
IP protocol for MAGED1 antibody 22053-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Clin Transl Med Saikosaponin-d impedes hippocampal neurogenesis and causes cognitive deficits by inhibiting the survival of neural stem/progenitor cells via neurotrophin receptor signaling in mice. | ||
Biochim Biophys Acta Mol Cell Biol Lipids Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system. | ||
Tumour Biol Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells.
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Gene NRAGE confers poor prognosis and promotes proliferation, invasion, and chemoresistance in gastric cancer.
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bioRxiv Histone chaperone HIRA, Promyelocytic Leukemia (PML) protein and p62/SQSTM1 coordinate to regulate inflammation during cell senescence |