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验证数据展示

  • WB analysis of Y79 using 12424-1-AP

    WB analysis of Y79 using 12424-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12424-1-AP (MAGEH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12424-1-AP

    IHC staining of human prostate cancer using 12424-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12424-1-AP (MAGEH1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 12424-1-AP

    IHC staining of human prostate cancer using 12424-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12424-1-AP (MAGEH1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of PC-3 using 12424-1-AP

    IF Staining of PC-3 using 12424-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 12424-1-AP (MAGEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAGEH1 Polyclonal antibody

MAGEH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12424-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Restin, Melanoma-associated antigen H1, MAGE-H1 antigen, APR-1, APR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, mouse brain tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12424-1-AP targets MAGEH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 MAGEH1 fusion protein Ag3090 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma antigen family H, 1
别名 Restin, Melanoma-associated antigen H1, MAGE-H1 antigen, APR-1, APR1
计算分子量 219 aa, 24 kDa
观测分子量 28 kDa
GenBank蛋白编号BC011954
基因名称 MAGEH1
Gene ID (NCBI) 28986
RRIDAB_2877856
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H213
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAGEH1 antibody 12424-1-APDownload protocol
IHC protocol for MAGEH1 antibody 12424-1-APDownload protocol
IF protocol for MAGEH1 antibody 12424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Oxid Med Cell Longev

DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway.

Authors - Yichen Zhao
View Article