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MAGEH1 Polyclonal antibody

MAGEH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12424-1-AP

Print datasheet

Synonyms

Restin, Melanoma-associated antigen H1, MAGE-H1 antigen, APR-1, APR1

验证数据展示

  • WB analysis of Y79 using 12424-1-AP

    WB analysis of Y79 using 12424-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12424-1-AP (MAGEH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12424-1-AP

    IHC staining of human prostate cancer using 12424-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12424-1-AP (MAGEH1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 12424-1-AP

    IHC staining of human prostate cancer using 12424-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12424-1-AP (MAGEH1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of PC-3 using 12424-1-AP

    IF Staining of PC-3 using 12424-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 12424-1-AP (MAGEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inY79 cells, mouse brain tissue, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12424-1-AP targets MAGEH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen MAGEH1 fusion protein Ag3090 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma antigen family H, 1
Synonyms Restin, Melanoma-associated antigen H1, MAGE-H1 antigen, APR-1, APR1
Calculated Molecular Weight 219 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC011954
Gene Symbol MAGEH1
Gene ID (NCBI) 28986
RRIDAB_2877856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H213
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAGEH1 antibody 12424-1-APDownload protocol
IHC protocol for MAGEH1 antibody 12424-1-APDownload protocol
IF protocol for MAGEH1 antibody 12424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Oxid Med Cell Longev

DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway.

Authors - Yichen Zhao
View Article