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验证数据展示

  • WB analysis using 25189-1-AP

    WB analysis using 25189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25189-1-AP (MAGI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-87 MG using 25189-1-AP

    IF Staining of U-87 MG using 25189-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using MAGI2 antibody (25189-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MAGI2 Polyclonal antibody

MAGI2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25189-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

ARIP1, AIP-1, AIP1, AIP 1, ACVRIP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inU-87 MG cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25189-1-AP targets MAGI2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAGI2 fusion protein Ag18279 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane associated guanylate kinase, WW and PDZ domain containing 2
别名 ARIP1, AIP-1, AIP1, AIP 1, ACVRIP1
计算分子量 1455 aa, 159 kDa
观测分子量 140 kDa
GenBank蛋白编号BC150277
基因名称 MAGI2
Gene ID (NCBI) 9863
RRIDAB_3085775
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UL8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI2.

实验方案

Product Specific Protocols
WB protocol for MAGI2 antibody 25189-1-APDownload protocol
IF protocol for MAGI2 antibody 25189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols