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Species Reactivity
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验证数据展示

  • WB analysis using 25189-1-AP

    WB analysis using 25189-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25189-1-AP (MAGI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-87 MG using 25189-1-AP

    IF Staining of U-87 MG using 25189-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using MAGI2 antibody (25189-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MAGI2 Polyclonal antibody

MAGI2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25189-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ARIP1, AIP-1, AIP1, AIP 1, ACVRIP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inU-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25189-1-AP targets MAGI2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAGI2 fusion protein Ag18279 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 2
Synonyms ARIP1, AIP-1, AIP1, AIP 1, ACVRIP1
Calculated Molecular Weight 1455 aa, 159 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC150277
Gene Symbol MAGI2
Gene ID (NCBI) 9863
RRIDAB_3085775
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UL8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI2.

实验方案

Product Specific Protocols
WB protocol for MAGI2 antibody 25189-1-APDownload protocol
IF protocol for MAGI2 antibody 25189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols