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验证数据展示

  • WB analysis using 22615-1-AP

    WB analysis using 22615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22615-1-AP (MAGI3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 22615-1-AP

    IHC staining of human ovary cancer using 22615-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22615-1-AP (MAGI3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAGI3 Polyclonal antibody

MAGI3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22615-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3, Membrane-associated guanylate kinase inverted 3, MAGI-3, KIAA1634

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22615-1-AP targets MAGI3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAGI3 fusion protein Ag18394 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane associated guanylate kinase, WW and PDZ domain containing 3
别名 Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3, Membrane-associated guanylate kinase inverted 3, MAGI-3, KIAA1634
计算分子量 1481 aa, 163 kDa
观测分子量140 kDa
GenBank蛋白编号BC130409
基因名称 MAGI3
Gene ID (NCBI) 260425
RRIDAB_3669402
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ5TCQ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI3.

实验方案

Product Specific Protocols
WB protocol for MAGI3 antibody 22615-1-APDownload protocol
IHC protocol for MAGI3 antibody 22615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols