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验证数据展示

  • WB analysis using 22615-1-AP

    WB analysis using 22615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22615-1-AP (MAGI3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 22615-1-AP

    IHC staining of human ovary cancer using 22615-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22615-1-AP (MAGI3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAGI3 Polyclonal antibody

MAGI3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22615-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3, Membrane-associated guanylate kinase inverted 3, MAGI-3, KIAA1634

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22615-1-AP targets MAGI3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAGI3 fusion protein Ag18394 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane associated guanylate kinase, WW and PDZ domain containing 3
Synonyms Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3, Membrane-associated guanylate kinase inverted 3, MAGI-3, KIAA1634
Calculated Molecular Weight 1481 aa, 163 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC130409
Gene Symbol MAGI3
Gene ID (NCBI) 260425
RRIDAB_3669402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ5TCQ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI3.

实验方案

Product Specific Protocols
WB protocol for MAGI3 antibody 22615-1-APDownload protocol
IHC protocol for MAGI3 antibody 22615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols