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验证数据展示

  • WB analysis using 68293-1-Ig

    WB analysis using 68293-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68293-1-Ig (MAGOH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

MAGOH Monoclonal antibody

MAGOH Monoclonal Antibody for WB, ELISA
Cat No. 68293-1-Ig

产品说明书

CloneNo. 2G6B9

宿主/亚型

Mouse / IgG1

种属反应性

Human, MOUSE, RAT, RABBIT

应用

WB, ELISA

别名

MAGOH, MAGOHA, Protein mago nashi homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy MAGOH Ready-To-Use IHC Kit. MAGOH primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68293-1-Ig targets MAGOH in WB, ELISA applications and shows reactivity with Human, MOUSE, RAT, RABBIT samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, MOUSE, RAT, RABBIT
免疫原 MAGOH fusion protein Ag3004 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mago-nashi homolog, proliferation-associated (Drosophila)
别名 MAGOH, MAGOHA, Protein mago nashi homolog
计算分子量 146 aa, 17 kDa
观测分子量17 kDa
GenBank蛋白编号BC018211
基因名称 MAGOH
Gene ID (NCBI) 4116
RRIDAB_2935373
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61326
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAGOH, belonging to the mago nashi family, is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and a core EJC component Y14.

实验方案

Product Specific Protocols
WB protocol for MAGOH antibody 68293-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols