MAGOH Monoclonal antibody
MAGOH Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, MOUSE, RAT, RABBIT
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2G6B9
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy MAGOH Ready-To-Use IHC Kit. MAGOH primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68293-1-Ig targets MAGOH in WB, ELISA applications and shows reactivity with Human, MOUSE, RAT, RABBIT samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, MOUSE, RAT, RABBIT |
| Immunogen | MAGOH fusion protein Ag3004 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mago-nashi homolog, proliferation-associated (Drosophila) |
| Synonyms | MAGOH, MAGOHA, Protein mago nashi homolog |
| Calculated Molecular Weight | 146 aa, 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC018211 |
| Gene Symbol | MAGOH |
| Gene ID (NCBI) | 4116 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P61326 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAGOH, belonging to the mago nashi family, is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and a core EJC component Y14.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAGOH antibody 68293-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |