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MAK16 Polyclonal antibody

MAK16 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17505-1-AP

Print datasheet

Synonyms

MAK16, MAK16 homolog (S. cerevisiae), MAK16L, NNP78, Protein MAK16 homolog, Protein RBM13, RBM13

验证数据展示

  • WB analysis using 17505-1-AP

    WB analysis using 17505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17505-1-AP (MAK16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 17505-1-AP

    IF Staining of HEK-293 using 17505-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 17505-1-AP (MAK16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17505-1-AP targets MAK16 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAK16 fusion protein Ag11705 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name MAK16 homolog (S. cerevisiae)
Synonyms MAK16, MAK16 homolog (S. cerevisiae), MAK16L, NNP78, Protein MAK16 homolog, Protein RBM13, RBM13
Calculated Molecular Weight 300 aa, 35 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC050528
Gene Symbol MAK16
Gene ID (NCBI) 84549
RRIDAB_2281504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAK16 antibody 17505-1-APDownload protocol
IF protocol for MAK16 antibody 17505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols