验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse heart tissue, Jurkat cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
CoIP | See 1 publications below |
产品信息
55493-1-AP targets MAML1 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, ELISA Application Description |
Cited Applications | WB, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mastermind-like 1 (Drosophila) |
Synonyms | KIAA0200, Mam 1, Mam1, MAML1, mastermind like 1 (Drosophila), Mastermind like protein 1 |
Calculated Molecular Weight | 108 kDa |
Observed Molecular Weight | 108-120 kDa |
GenBank Accession Number | NM_014757 |
Gene Symbol | MAML1 |
Gene ID (NCBI) | 9794 |
RRID | AB_11182286 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q92585 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for MAML1 antibody 55493-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |