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验证数据展示

  • WB analysis of mouse heart using 55493-1-AP

    WB analysis of mouse heart using 55493-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 55493-1-AP

    WB analysis using 55493-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MAML1 Polyclonal antibody

MAML1 Polyclonal Antibody for WB, ELISA
Cat No. 55493-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA and More (1)

Synonyms

KIAA0200, Mam 1, Mam1, MAML1, mastermind like 1 (Drosophila), Mastermind like protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55493-1-AP targets MAML1 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mastermind-like 1 (Drosophila)
Synonyms KIAA0200, Mam 1, Mam1, MAML1, mastermind like 1 (Drosophila), Mastermind like protein 1
Calculated Molecular Weight 108 kDa
Observed Molecular Weight 108-120 kDa
GenBank Accession NumberNM_014757
Gene Symbol MAML1
Gene ID (NCBI) 9794
RRIDAB_11182286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa.

实验方案

Product Specific Protocols
WB protocol for MAML1 antibody 55493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Int J Biol Sci

Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling.

Authors - Yanzhu Shi
View Article