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MAN1A1 Polyclonal antibody

MAN1A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 23382-1-AP

Print datasheet

Synonyms

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA, Man9-mannosidase, Man(9)-alpha-mannosidase, EC:3.2.1.113, Alpha-1,2-mannosidase IA

验证数据展示

  • WB analysis using 23382-1-AP

    WB analysis using 23382-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23382-1-AP (MAN1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 23382-1-AP

    IP experiment of MCF-7 using 23382-1-AP

    IP result of anti-MAN1A1 (IP:23382-1-AP, 4ug; Detection:23382-1-AP 1:300) with MCF-7 cells lysate 2000ug.

  • FC experiment of HepG2 using 23382-1-AP

    FC experiment of HepG2 using 23382-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MAN1A1 Polyclonal antibody (23382-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human ovary cancer using 23382-1-AP

    IHC staining of human ovary cancer using 23382-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23382-1-AP (MAN1A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 23382-1-AP

    IF Staining of HepG2 using 23382-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (23382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23382-1-AP targets MAN1A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAN1A1 fusion protein Ag20060 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannosidase, alpha, class 1A, member 1
Synonyms Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA, Man9-mannosidase, Man(9)-alpha-mannosidase, EC:3.2.1.113, Alpha-1,2-mannosidase IA
Calculated Molecular Weight 653 aa, 73 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC065827
Gene Symbol MAN1A1
Gene ID (NCBI) 4121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).

实验方案

Product Specific Protocols
WB protocol for MAN1A1 antibody 23382-1-APDownload protocol
IHC protocol for MAN1A1 antibody 23382-1-APDownload protocol
IF protocol for MAN1A1 antibody 23382-1-APDownload protocol
IP protocol for MAN1A1 antibody 23382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols