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MAN1B1 Monoclonal antibody, PBS Only

MAN1B1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C9F10

Cat no : 68121-1-PBS

Print datasheet

Synonyms

ER alpha 1,2 mannosidase, ER mannosidase 1, ERMan1, MAN1B1, MANA ER

验证数据展示

  • WB analysis of HeLa using 68121-1-Ig (same clone as 68121-1-PBS)

    WB analysis of HeLa using 68121-1-Ig (same clone as 68121-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

  • WB analysis using 68121-1-Ig (same clone as 68121-1-PBS)

    WB analysis using 68121-1-Ig (same clone as 68121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

  • WB analysis using 68121-1-Ig (same clone as 68121-1-PBS)

    WB analysis using 68121-1-Ig (same clone as 68121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68121-1-Ig (MAN1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68121-1-Ig (same clone as 68121-1-PBS)

    IF Staining of U2OS using 68121-1-Ig (same clone as 68121-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using MAN1B1 antibody (68121-1-Ig, Clone: 3C9F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68121-1-PBS in a different storage buffer formulation.

产品信息

68121-1-PBS targets MAN1B1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen MAN1B1 fusion protein Ag28302 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name mannosidase, alpha, class 1B, member 1
Synonyms ER alpha 1,2 mannosidase, ER mannosidase 1, ERMan1, MAN1B1, MANA ER
Calculated Molecular Weight 80 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC002953
Gene Symbol MAN1B1
Gene ID (NCBI) 11253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKM7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAN1B1, also known as ERMAN1, MANA-ER, belongs to the glycosyl hydrolase 47 family. MAN1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation, specifically converting Man9GlcNAc to Man8GlcNAc isomer B. At high enzyme concentrations, it further trims the carbohydrates to Man5-6GlcNAc2 (PMID: 18003979). Mutations in MAN1B1 cause autosomal-recessive intellectual disability. This gene has been associated with Rafiq syndrome (PMID: 21763484).