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MAN2A1 Polyclonal antibody

MAN2A1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21132-1-AP

Print datasheet

Synonyms

Alpha mannosidase 2, AMan II, Golgi alpha mannosidase II, GOLIM7, Man II, MAN2A1, MANA2, MANII

验证数据展示

  • IHC staining of human stomach using 21132-1-AP

    IHC staining of human stomach using 21132-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human stomach using 21132-1-AP

    IHC staining of human stomach using 21132-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 21132-1-AP

    IF Staining of HeLa using 21132-1-AP

    Immunofluorescent analysis of HeLa cells using 21132-1-AP (MAN2A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse stomach using 21132-1-AP

    IHC staining of mouse stomach using 21132-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse stomach using 21132-1-AP

    IHC staining of mouse stomach using 21132-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:500 (under 10x lens).

经过测试的应用

Positive IHC detected inhuman stomach tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21132-1-AP targets MAN2A1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MAN2A1 fusion protein Ag15536 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannosidase, alpha, class 2A, member 1
Synonyms Alpha mannosidase 2, AMan II, Golgi alpha mannosidase II, GOLIM7, Man II, MAN2A1, MANA2, MANII
Calculated Molecular Weight 1144 aa, 131 kDa
GenBank Accession NumberBC142656
Gene Symbol MAN2A1
Gene ID (NCBI) 4124
RRIDAB_2878816
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16706
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MAN2A1 antibody 21132-1-APDownload protocol
IF protocol for MAN2A1 antibody 21132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols