验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19189-1-AP targets MAN2C1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | MAN2C1 fusion protein Ag6284 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mannosidase, alpha, class 2C, member 1 |
| 别名 | Alpha mannosidase 2C1, Alpha mannosidase 6A8B, MAN2C1, MAN6A8, MANA, MANA1 |
| 计算分子量 | 116 kDa |
| 观测分子量 | 116 kDa |
| GenBank蛋白编号 | BC050550 |
| 基因名称 | MAN2C1 |
| Gene ID (NCBI) | 4123 |
| RRID | AB_3669307 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NTJ4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha mannosidase 2C1(MAN2C1) an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol. MAN2C1 cleaves alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues on cytoplasmatic free oligosaccharides generated by N-glycoprotein degradation pathways (PMID: 16848760). Impaired catabolism of free oligosaccharides due to MAN2C1 variants causes a neurodevelopmental disorder (PMID: 35045343).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAN2C1 antibody 19189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |