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MANEA Polyclonal antibody

MANEA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21296-1-AP

Print datasheet

Synonyms

ENDO, Endo alpha mannosidase, Endomannosidase, hEndo, Mandaselin, MANEA, mannosidase, endo alpha

验证数据展示

  • WB analysis of HEK-293 using 21296-1-AP

    WB analysis of HEK-293 using 21296-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 21296-1-AP

    IHC staining of human breast cancer using 21296-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IHC staining of human breast cancer using 21296-1-AP

    IHC staining of human breast cancer using 21296-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IF Staining of HEK-293 using 21296-1-AP

    IF Staining of HEK-293 using 21296-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21296-1-AP (MANEA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21296-1-AP is MANEA Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MANEA fusion protein Ag15847 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mannosidase, endo-alpha
Synonyms ENDO, Endo alpha mannosidase, Endomannosidase, hEndo, Mandaselin, MANEA, mannosidase, endo alpha
Calculated Molecular Weight 462 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC146671
Gene Symbol MANEA
Gene ID (NCBI) 79694
RRIDAB_2878836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SRI9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MANEA antibody 21296-1-APDownload protocol
IHC protocol for MANEA antibody 21296-1-APDownload protocol
IF protocol for MANEA antibody 21296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols