• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293 using 21296-1-AP

    WB analysis of HEK-293 using 21296-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21296-1-AP (MANEA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 21296-1-AP

    IHC staining of human breast cancer using 21296-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IHC staining of human breast cancer using 21296-1-AP

    IHC staining of human breast cancer using 21296-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21296-1-AP (MANEA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • IF Staining of HEK-293 using 21296-1-AP

    IF Staining of HEK-293 using 21296-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MANEA antibody (21296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MANEA Polyclonal antibody

MANEA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Glycoprotein endo-alpha-1,2-mannosidase, Endomannosidase, Endo-alpha mannosidase, Endo alpha mannosidase, ENDO

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21296-1-AP targets MANEA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MANEA fusion protein Ag15847 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosidase, endo-alpha
别名 Glycoprotein endo-alpha-1,2-mannosidase, Endomannosidase, Endo-alpha mannosidase, Endo alpha mannosidase, ENDO
计算分子量 462 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC146671
基因名称 MANEA
Gene ID (NCBI) 79694
RRIDAB_2878836
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5SRI9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MANEA antibody 21296-1-APDownload protocol
IHC protocol for MANEA antibody 21296-1-APDownload protocol
IF protocol for MANEA antibody 21296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols