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MAP1B Polyclonal antibody
MAP1B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse cerebellum tissue, human brain tissue |
Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | SH-SY5Y cells |
Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21633-1-AP targets MAP1B in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | MAP1B fusion protein Ag16255 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | microtubule-associated protein 1B |
Synonyms | Microtubule-associated protein 1B, MAP1B heavy chain, MAP-1B, MAP1 light chain LC1 |
Calculated Molecular Weight | 2468 aa, 271 kDa |
Observed Molecular Weight | 320 kDa |
GenBank Accession Number | BC141853 |
Gene Symbol | MAP1B |
Gene ID (NCBI) | 4131 |
RRID | AB_10793666 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P46821 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for MAP1B antibody 21633-1-AP | Download protocol |
IHC protocol for MAP1B antibody 21633-1-AP | Download protocol |
IF protocol for MAP1B antibody 21633-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation | ||
EMBO Rep MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.
| ||
Cereb Cortex eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures. | ||
Front Cell Neurosci Endothelin-1, over-expressed in SOD1G93A mice, aggravates injury of NSC34-hSOD1G93A cells through complicated molecular mechanism revealed by quantitative proteomics analysis | ||
Hum Mol Genet Microtubule associated protein 1B dysregulates microtubule dynamics and neuronal mitochondrial transport in Spinal Muscular Atrophy. | ||
Cell Cycle Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability. |