验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, A431 cells, HepG2 cells, MCF-7 cells, HeLa cells, A549 cells, Jurkat cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
68574-1-Ig targets MAP2K3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | Human, mouse, rat |
文献引用反应性 | human |
免疫原 | MAP2K3 fusion protein Ag4861 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | mitogen-activated protein kinase kinase 3 |
别名 | MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MAPKK3, MEK 3, MEK3, MKK3, PRKMK3 |
计算分子量 | 347 aa, 39 kDa |
观测分子量 | 36-40 kDa |
GenBank蛋白编号 | BC032478 |
基因名称 | MAP2K3 |
Gene ID (NCBI) | 5606 |
RRID | AB_3670384 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P46734 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for MAP2K3 antibody 68574-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Ethnopharmacol Luteolin and its analog luteolin-7-methylether from Leonurus japonicus Houtt suppress aromatase-mediated estrogen biosynthesis to alleviate polycystic ovary syndrome by the inhibition of tumor progression locus 2 |