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MAP2K3 Monoclonal antibody

MAP2K3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H3E10

Cat no : 68574-1-Ig

Print datasheet

Synonyms

MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MAPKK3, MEK 3, MEK3, MKK3, PRKMK3

验证数据展示

  • WB analysis using 68574-1-Ig

    WB analysis using 68574-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68574-1-Ig (MAP2K3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68574-1-Ig

    WB analysis of A431 using 68574-1-Ig

    WB result of MAP2K3 antibody (68574-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected A431 cells.

经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, HepG2 cells, MCF-7 cells, HeLa cells, A549 cells, Jurkat cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68574-1-Ig targets MAP2K3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen MAP2K3 fusion protein Ag4861 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 3
Synonyms MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MAPKK3, MEK 3, MEK3, MKK3, PRKMK3
Calculated Molecular Weight 347 aa, 39 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC032478
Gene Symbol MAP2K3
Gene ID (NCBI) 5606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP46734
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAP2K3 antibody 68574-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Ethnopharmacol

Luteolin and its analog luteolin-7-methylether from Leonurus japonicus Houtt suppress aromatase-mediated estrogen biosynthesis to alleviate polycystic ovary syndrome by the inhibition of tumor progression locus 2

Authors - Xiao-Ke Shi
View Article