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验证数据展示

  • WB analysis of mouse brain using 51142-1-AP

    WB analysis of mouse brain using 51142-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51142-1-AP (MAP2K4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 51142-1-AP

    IHC staining of human breast cancer using 51142-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 51142-1-AP

    IHC staining of human breast cancer using 51142-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51142-1-AP (MAP2K4 antibody at dilution of 1:50 (under 40x lens).

MAP2K4 Polyclonal antibody

MAP2K4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51142-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51142-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 4
别名 JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1
计算分子量 399 aa, 44 kDa
观测分子量44-50 kDa
GenBank蛋白编号BC036032
基因名称 MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2881252
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP45985
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAP2K4 antibody 51142-1-APDownload protocol
IHC protocol for MAP2K4 antibody 51142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols