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MAP2K4 Monoclonal antibody

MAP2K4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E4C1

Cat no : 67333-1-Ig

Print datasheet

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

验证数据展示

  • WB analysis using 67333-1-Ig

    WB analysis using 67333-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67333-1-Ig (MAP2K4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67333-1-Ig

    IHC staining of human breast cancer using 67333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67333-1-Ig

    IHC staining of human breast cancer using 67333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67333-1-Ig targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, rat
Cited Reactivityhuman, mouse
Immunogen MAP2K4 fusion protein Ag11348 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 4
Synonyms JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1
Calculated Molecular Weight 399 aa, 44 kDa
Observed Molecular Weight44-50 kDa
GenBank Accession NumberBC036032
Gene Symbol MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2882591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45985
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for MAP2K4 antibody 67333-1-IgDownload protocol
IHC protocol for MAP2K4 antibody 67333-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PNAS Nexus

A potent tumor-selective ERK pathway inactivator with high therapeutic index.

Authors - Zehua Zuo
View Article
mouseWB

Phytomedicine

Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity

Authors - Zhong Xian
View Article