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  • Featured Product
  • KD/KO Validated

MAP3K1 Polyclonal antibody

MAP3K1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19970-1-AP

Print datasheet

Synonyms

MEKK, MEK kinase 1, MAPKKK1, MAPK/ERK kinase kinase 1, EC:2.7.11.25

验证数据展示

  • WB analysis using 19970-1-AP

    WB analysis using 19970-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 19970-1-AP

    IP experiment of NIH/3T3 using 19970-1-AP

    IP result of anti-MAP3K1 (IP:19970-1-AP, 4ug; Detection:19970-1-AP 1:1000) with NIH/3T3 cells lysate 1120 ug.

  • WB analysis using 19970-1-AP

    WB analysis using 19970-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19970-1-AP (MAP3K1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 19970-1-AP

    IHC staining of human spleen using 19970-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human spleen using 19970-1-AP

    IHC staining of human spleen using 19970-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19970-1-AP (MAP3K1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 19970-1-AP

    IF Staining of HeLa using 19970-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19970-1-AP (MAP3K1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse embryo tissue, A431 cells, mouse spleen tissue, A549 cells, NIH/3T3 cells, rat spleen tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19970-1-AP targets MAP3K1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 1
Synonyms MEKK, MEK kinase 1, MAPKKK1, MAPK/ERK kinase kinase 1, EC:2.7.11.25
Calculated Molecular Weight 164 kDa
Observed Molecular Weight 164 kDa
GenBank Accession NumberNM_005921
Gene Symbol MAP3K1
Gene ID (NCBI) 4214
RRIDAB_2878633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13233
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K1, also named as MAPKKK1, MEKK and MEKK1, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K1 is a component of a protein kinase signal transduction cascade. MAP3K1 activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. It activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K1.

实验方案

Product Specific Protocols
WB protocol for MAP3K1 antibody 19970-1-APDownload protocol
IHC protocol for MAP3K1 antibody 19970-1-APDownload protocol
IF protocol for MAP3K1 antibody 19970-1-APDownload protocol
IP protocol for MAP3K1 antibody 19970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol Res

Circulating miR-320b Contributes to CD4+ T-Cell Proliferation in Systemic Lupus Erythematosus via MAP3K1

Authors - Zutong Li
View Article
humanWB

Int J Biol Sci

LncRNA SLCO4A1-AS1 predicts poor prognosis and promotes proliferation and metastasis via the EGFR/MAPK pathway in colorectal cancer.

Authors - Rui Tang
View Article
humanWB

Int Immunopharmacol

Extracellular vesicles secreted from mesenchymal stem cells exert anti-apoptotic and anti-inflammatory effects via transmitting microRNA-18b in rats with diabetic retinopathy.

Authors - Zepeng Xu
View Article
mouseWB,IF

Biomed Pharmacother

Xihuang pill promotes apoptosis of Treg cells in the tumor microenvironment in 4T1 mouse breast cancer by upregulating MEKK1/SEK1/JNK1/AP-1 pathway.

Authors - Liang Su
View Article
humanWB

Cell Death Dis

LncRNA KCNQ1OT1 activated by c-Myc promotes cell proliferation via interacting with FUS to stabilize MAP3K1 in acute promyelocytic leukemia.

Authors - Doudou Tang
View Article
mouseWB

Clin Sci (Lond)

USP4 deficiency exacerbates hepatic ischaemia/reperfusion injury via TAK1 signalling.

Authors - Jiangqiao Zhou
View Article