• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MLK2 Polyclonal antibody

MLK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19974-1-AP

Print datasheet

Synonyms

MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST

验证数据展示

  • WB analysis of human skeletal muscle using 19974-1-AP

    WB analysis of human skeletal muscle using 19974-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19974-1-AP (MLK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 19974-1-AP

    IHC staining of human pancreas cancer using 19974-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19974-1-AP (MLK2 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas cancer using 19974-1-AP

    IHC staining of human pancreas cancer using 19974-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19974-1-AP (MLK2 antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, human brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19974-1-AP targets MLK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 10
Synonyms MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST
Calculated Molecular Weight 104 kDa
Observed Molecular Weight 104-115 kDa
GenBank Accession NumberNM_002446
Gene Symbol MAP3K10
Gene ID (NCBI) 4294
RRIDAB_10732824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02779
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K10, also named as MLK2 and MST, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K10 activates the JUN N-terminal pathway. MAP3K10 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes the N-term of MAP3K10. The antibody has no cross reaction to MAP3K9.

实验方案

Product Specific Protocols
WB protocol for MLK2 antibody 19974-1-APDownload protocol
IHC protocol for MLK2 antibody 19974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Lab Chip

Paper-based microreactor array for rapid screening of cell signaling cascades.

Authors - Chia-Hao Huang
View Article
humanWB

J Biol Chem

Phosphorylation of mixed lineage kinase MLK3 by cyclin-dependent kinases CDK1 and CDK2 controls ovarian cancer cell division.

Authors - Luis Cedeno-Rosario
View Article