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MAP3K10 Polyclonal antibody

MAP3K10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29985-1-AP

Print datasheet

Synonyms

MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST

验证数据展示

  • WB analysis using 29985-1-AP

    WB analysis using 29985-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29985-1-AP (MAP3K10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29985-1-AP

    WB analysis using 29985-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29985-1-AP (MAP3K10 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29985-1-AP targets MAP3K10 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MAP3K10 fusion protein Ag32419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 10
Synonyms MAP3K10, Mixed lineage kinase 2, MLK2, MST, Protein kinase MST
Calculated Molecular Weight104KD
Observed Molecular Weight104-115 kDa
GenBank Accession NumberNM_002446
Gene Symbol MAP3K10
Gene ID (NCBI) 4294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02779
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K10, also named as MLK2 and MST, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAP kinase kinase kinase subfamily. MAP3K10 activates the JUN N-terminal pathway. MAP3K10 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. MAP3K10 can be detected 104-115 kDa by phosphorylation (PMID: 23178452).

实验方案

Product Specific Protocols
WB protocol for MAP3K10 antibody 29985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols