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验证数据展示

  • WB analysis using 29265-1-AP

    WB analysis using 29265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29265-1-AP (MEKK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 29265-1-AP

    IHC staining of human breast cancer using 29265-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29265-1-AP (MEKK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 29265-1-AP

    IHC staining of human breast cancer using 29265-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29265-1-AP (MEKK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MEKK2 Polyclonal antibody

MEKK2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29265-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MAP3K2, EC:2.7.11.25, MEKK2B, MEKK 2, MEK kinase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29265-1-AP targets MEKK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 MEKK2 fusion protein Ag30886 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 2
别名 MAP3K2, EC:2.7.11.25, MEKK2B, MEKK 2, MEK kinase 2
计算分子量 70 kDa
观测分子量70 kDa
GenBank蛋白编号NM_006609
基因名称 MAP3K2
Gene ID (NCBI) 10746
RRIDAB_2918263
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2U5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K2, also named as MEKK2 and MEKK2B, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAPK kinase kinase subfamily. It is a component of a protein kinase signal transduction cascade. MAP3K2 mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. The antibody is specific to MAP3K2.

实验方案

Product Specific Protocols
WB protocol for MEKK2 antibody 29265-1-APDownload protocol
IHC protocol for MEKK2 antibody 29265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

Concurrent targeting of MAP3K3 and BRD4 by miR-3140-3p overcomes acquired resistance to BET inhibitors in neuroblastoma cells.

Authors - Chang Liu
View Article