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验证数据展示

  • WB analysis of K-562 using 21610-1-AP

    WB analysis of K-562 using 21610-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21610-1-AP (MAP3K4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21610-1-AP

    IF Staining of HeLa using 21610-1-AP

    Immunofluorescent analysis of Hela cells, using MAP3K4 antibody 21610-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

MAP3K4 Polyclonal antibody

MAP3K4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21610-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

别名

KIAA0213, MAP three kinase 1, MAP3K4, MAPK/ERK kinase kinase 4, MAPKKK4, MEK kinase 4, MEKK 4, MEKK4, MTK1, PRO0412

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21610-1-AP targets MAP3K4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 MAP3K4 fusion protein Ag16254 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 4
别名 KIAA0213, MAP three kinase 1, MAP3K4, MAPK/ERK kinase kinase 4, MAPKKK4, MEK kinase 4, MEKK 4, MEKK4, MTK1, PRO0412
计算分子量 1608 aa, 182 kDa
观测分子量 170-180 kDa
GenBank蛋白编号BC146770
基因名称 MAP3K4
Gene ID (NCBI) 4216
RRIDAB_10804775
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6R4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAP3K4 antibody 21610-1-APDownload protocol
IF protocol for MAP3K4 antibody 21610-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Gene Med

Construction and validation of molecular subtype and signature of immune cell-related telomeric genes and prediction of prognosis and immunotherapy efficacy in ovarian cancer patients

Authors - Lele Ling
View Article
mouseWB

J Adv Res

Hypoxia-induced degradation of FTO promotes apoptosis by unmasking RACK1-mediated activation of MTK1-JNK1/2 pathway

Authors - Chengyu Li
View Article