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MAP3K6 Polyclonal antibody

MAP3K6 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16212-1-AP

Print datasheet

Synonyms

Mitogen-activated protein kinase kinase kinase 6, MEKK6, MAPKKK6, EC:2.7.11.25, ASK2

验证数据展示

  • IHC staining of human liver using 16212-1-AP

    IHC staining of human liver using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 16212-1-AP

    IHC staining of human liver using 16212-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16212-1-AP (MAP3K6 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 16212-1-AP

    IF Staining of HeLa using 16212-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MAP3K6 antibody (16212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16212-1-AP targets MAP3K6 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAP3K6 fusion protein Ag9206 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 6
Synonyms Mitogen-activated protein kinase kinase kinase 6, MEKK6, MAPKKK6, EC:2.7.11.25, ASK2
Calculated Molecular Weight1148aa,128 kDa; 1011aa,113 kDa; 797aa,89 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC015914
Gene Symbol MAP3K6
Gene ID (NCBI) 9064
RRIDAB_2878229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95382
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MAP3K6 antibody 16212-1-APDownload protocol
IF protocol for MAP3K6 antibody 16212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols