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TAK1 Polyclonal antibody

TAK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29167-1-AP

Print datasheet

Synonyms

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a

验证数据展示

  • WB analysis using 29167-1-AP

    WB analysis using 29167-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29167-1-AP (TAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29167-1-AP

    IHC staining of human prostate cancer using 29167-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29167-1-AP (TAK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAP3K7/TAK1 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29167-1-AP targets TAK1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen TAK1 fusion protein Ag30725 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 7
Synonyms MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a
Calculated Molecular Weight 579 aa, 64 kDa
Observed Molecular Weight64-70 kDa
GenBank Accession NumberBC017715
Gene Symbol TAK1
Gene ID (NCBI) 6885
RRIDAB_2918242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for TAK1 antibody 29167-1-APDownload protocol
IHC protocol for TAK1 antibody 29167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols