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验证数据展示

  • IP experiment of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

    IP experiment of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

    IP result of anti-MAP4 (IP:11229-1-AP, 4ug; Detection:11229-1-AP 1:1000) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • WB analysis using 11229-1-AP (same clone as 11229-1-PBS)

    WB analysis using 11229-1-AP (same clone as 11229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MAP4 Polyclonal antibody, PBS Only

MAP4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11229-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

11229-1-PBS targets MAP4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAP4 fusion protein Ag1741 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule-associated protein 4
别名
计算分子量 121 kDa
观测分子量 210-240 kDa
GenBank蛋白编号BC012794
基因名称 MAP4
Gene ID (NCBI) 4134
RRIDAB_2140681
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27816
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)