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  • Featured Product
  • KD/KO Validated

MAP4 Polyclonal antibody, PBS Only

MAP4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11229-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IP experiment of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

    IP experiment of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

    IP result of anti-MAP4 (IP:11229-1-AP, 4ug; Detection:11229-1-AP 1:1000) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

  • WB analysis using 11229-1-AP (same clone as 11229-1-PBS)

    WB analysis using 11229-1-AP (same clone as 11229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

产品信息

11229-1-PBS targets MAP4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAP4 fusion protein Ag1741 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microtubule-associated protein 4
Synonyms
Calculated Molecular Weight 121 kDa
Observed Molecular Weight 210-240 kDa
GenBank Accession NumberBC012794
Gene Symbol MAP4
Gene ID (NCBI) 4134
RRIDAB_2140681
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27816
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)