• Featured Product
  • KD/KO Validated

MAP4K4 Polyclonal antibody

MAP4K4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 55247-1-AP

Print datasheet

Synonyms

FLH21957, HGK, HPK/GCK like kinase HGK, KIAA0687, MAP4K4, MEK kinase kinase 4, MEKKK 4, Nck interacting kinase, NIK



经过测试的应用

Positive WB detected inJurkat cells, PC-3 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skeletal muscle tissue, human placenta tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAP4K4 Ready-To-Use IHC Kit. MAP4K4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55247-1-AP targets MAP4K4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase kinase 4
Synonyms FLH21957, HGK, HPK/GCK like kinase HGK, KIAA0687, MAP4K4, MEK kinase kinase 4, MEKKK 4, Nck interacting kinase, NIK
Calculated Molecular Weight 151 kDa
Observed Molecular Weight 130-160 kDa
GenBank Accession NumberNM_004834
Gene Symbol MAP4K4
Gene ID (NCBI) 9448
RRIDAB_10836939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95819
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.

实验方案

Product Specific Protocols
WB protocol for MAP4K4 antibody 55247-1-APDownload protocol
IHC protocol for MAP4K4 antibody 55247-1-APDownload protocol
IP protocol for MAP4K4 antibody 55247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Phytomedicine

Artesunate improves glucose and lipid metabolism in db/db mice by regulating the metabolic profile and the MAPK/PI3K/Akt signalling pathway

Authors - Lulu Chen
humanWB

Anal Chem

Parallel-Reaction-Monitoring-Based Proteome-Wide Profiling of Differential Kinase Protein Expression during Prostate Cancer Metastasis in Vitro.

Authors - Weili Miao
humanWB

EBioMedicine

Targeting miR-30d reverses pathological cardiac hypertrophy.

Authors - Jin Li
mouseWB

J Proteome Res

Global Phosphoproteomic Analysis Reveals Significant Metabolic Reprogramming in the Termination of Liver Regeneration in Mice.

Authors - Jingzi Zhang
mouseWB,IF

Neural Regen Res

MAP4K4 induces early blood-brain barrier damage in a murine subarachnoid hemorrhage model.

Authors - Zheng Zou
  • KD Validated
humanIHC

PeerJ

Construction and validation of a novel prognostic signature of microRNAs in lung adenocarcinoma.

Authors - Wanzhen Li