• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MAP4K5 Polyclonal antibody

MAP4K5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12827-1-AP

Print datasheet

Synonyms

GCKR, KHS, KHS1, MAP4K5, MAPKKKK5, MEK kinase kinase 5, MEKKK 5

验证数据展示

  • WB analysis of mouse ovary using 12827-1-AP

    WB analysis of mouse ovary using 12827-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12827-1-AP (MAP4K5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 12827-1-AP

    IHC staining of human ovary tumor using 12827-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12827-1-AP (MAP4K5 antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inmouse ovary tissue, HeLa cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12827-1-AP targets MAP4K5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MAP4K5 fusion protein Ag3547 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase kinase 5
Synonyms GCKR, KHS, KHS1, MAP4K5, MAPKKKK5, MEK kinase kinase 5, MEKKK 5
Calculated Molecular Weight 846 aa, 95 kDa
Observed Molecular Weight 58 kDa, 95 kDa, 120 kDa
GenBank Accession NumberBC036013
Gene Symbol MAP4K5
Gene ID (NCBI) 11183
RRIDAB_2140986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4K4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAP4K5 antibody 12827-1-APDownload protocol
IHC protocol for MAP4K5 antibody 12827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols