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验证数据展示

  • IHC staining of mouse brain using 26078-1-AP

    IHC staining of mouse brain using 26078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26078-1-AP (MAP9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAP9 antibody included
  • IHC staining of mouse brain using 26078-1-AP

    IHC staining of mouse brain using 26078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26078-1-AP (MAP9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAP9 antibody included
  • WB analysis of SH-SY5Y using 26078-1-AP

    WB analysis of SH-SY5Y using 26078-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26078-1-AP

    IF Staining of HeLa using 26078-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MAP9 antibody (26078-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MAP9 Polyclonal antibody

MAP9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26078-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Microtubule-associated protein 9, Aster-associated protein, Aster associated protein, ASAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, K-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAP9 Ready-To-Use IHC Kit. MAP9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26078-1-AP targets MAP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性human, mouse, canine
免疫原 MAP9 fusion protein Ag23446 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microtubule-associated protein 9
别名 Microtubule-associated protein 9, Aster-associated protein, Aster associated protein, ASAP
观测分子量 38-42 kDa
GenBank蛋白编号BC146864
基因名称 MAP9
Gene ID (NCBI) 79884
RRIDAB_2880366
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ49MG5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAP9 antibody 26078-1-APDownload protocol
IHC protocol for MAP9 antibody 26078-1-APDownload protocol
IF protocol for MAP9 antibody 26078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineIF

Front Cell Neurosci

Molecular characterization of MAP9 in the photoreceptor sensory cilia as a modifier in canine RPGRIP1-associated cone-rod dystrophy

Authors - Kei Takahashi
View Article
humanIHC,IF

Dev Cell

MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement

Authors - Michael V Tran
View Article
mouseIF

EMBO J

Glutamylation imbalance impairs the molecular architecture of the photoreceptor cilium

Authors - Olivier Mercey
View Article