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JNK Polyclonal antibody

JNK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, canine and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28007-1-AP

Print datasheet

Synonyms

c Jun N terminal kinase 3, FLJ12099, FLJ33785, JNK, JNK1, JNK1/2/3, JNK2, JNK3, JNK3A, MAP kinase 10, MAP kinase p49 3F12, MAPK 10, MAPK10, MAPK8, MAPK8/9/10, MAPK9, p493F12, p54bSAPK, PRKM10

验证数据展示

  • WB analysis using 28007-1-AP

    WB analysis using 28007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28007-1-AP (JNK1/2/3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28007-1-AP

    IHC staining of mouse brain using 28007-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28007-1-AP (JNK1/2/3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK10 antibody included

经过测试的应用

Positive WB detected inNeuro-2a cells, MDCK cells, mouse brain tissue, mouse cerebellum tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAPK10 Ready-To-Use IHC Kit. MAPK10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28007-1-AP targets JNK in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, canine samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, canine
Cited Reactivitymouse
Immunogen JNK fusion protein Ag27823 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 10
Synonyms c Jun N terminal kinase 3, FLJ12099, FLJ33785, JNK, JNK1, JNK1/2/3, JNK2, JNK3, JNK3A, MAP kinase 10, MAP kinase p49 3F12, MAPK 10, MAPK10, MAPK8, MAPK8/9/10, MAPK9, p493F12, p54bSAPK, PRKM10
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 45-48 kDa, 54-57 kDa
GenBank Accession NumberBC065516
Gene Symbol JNK3
Gene ID (NCBI) 5602
RRIDAB_2881035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53779
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

JNK, also named MAPK10 (mitogen-activated protein kinase 10), belongs to MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is mainly expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. JNK has 3 isoforms of 48-53 kDa.

实验方案

Product Specific Protocols
WB protocol for JNK antibody 28007-1-APDownload protocol
IHC protocol for JNK antibody 28007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Biol Macromol

VSIG4 inhibits RANKL-induced osteoclastogenesis by enhancing Nrf2-dependent antioxidant response against reactive oxygen species production

Authors - Jiansen Miao
View Article