- Featured Product
- KD/KO Validated
MAPK12 Polyclonal antibody
MAPK12 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse heart tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy MAPK12 Ready-To-Use IHC Kit. MAPK12 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 3 publications below |
产品信息
The immunogen of 20184-1-AP is MAPK12 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | MAPK12 fusion protein Ag14073 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase 12 |
| Synonyms | ERK 6, ERK3, ERK6, MAP kinase 12, MAP kinase p38 gamma, MAPK 12, MAPK12, P38GAMMA, PRKM12, SAPK 3, SAPK3 |
| Calculated Molecular Weight | 367 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC015741 |
| Gene Symbol | MAPK12 |
| Gene ID (NCBI) | 6300 |
| RRID | AB_10665949 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53778 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAPK12 antibody 20184-1-AP | Download protocol |
| IHC protocol for MAPK12 antibody 20184-1-AP | Download protocol |
| IP protocol for MAPK12 antibody 20184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Tumour Biol Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.
| ||
BMC Endocr Disord The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas. | ||
Clin Transl Oncol A retrospective analysis of the clinicopathological features and prognostic value of MAPK12 protein expression in diffuse large B-cell lymphoma | ||
J Orthop Translat Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis |
