• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Ramos using 60252-1-Ig

    WB analysis of Ramos using 60252-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 60252-1-Ig

    WB analysis of HepG2 using 60252-1-Ig

    WB result of MAPK13 antibody (60252-1-Ig, 1:2000) with si-Control and si-MAPK13 transfected HepG2 cells.

  • IHC staining of human pancreas cancer using 60252-1-Ig

    IHC staining of human pancreas cancer using 60252-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60252-1-Ig (MAPK13 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK13 antibody included
  • Featured Product
  • KD/KO验证

MAPK13 Monoclonal antibody

MAPK13 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60252-1-Ig

产品说明书

CloneNo. 1B12A6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inRamos cells, HepG2 cells, Daudi cells, HEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAPK13 Ready-To-Use IHC Kit. MAPK13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60252-1-Ig targets MAPK13 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 MAPK13 fusion protein Ag21240 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 13
别名 MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4
计算分子量 38 kDa
观测分子量38-42 kDa
GenBank蛋白编号BC000433
基因名称 MAPK13
Gene ID (NCBI) 5603
RRIDAB_2881373
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15264
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAPK13(Mitogen-activated protein kinase 13) is also named as PRKM13, SAPK4 and belongs to the protein kinase superfamily. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2.

实验方案

Product Specific Protocols
WB protocol for MAPK13 antibody 60252-1-IgDownload protocol
IHC protocol for MAPK13 antibody 60252-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols