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MAPK15 Polyclonal antibody

MAPK15 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13452-1-AP

Print datasheet

Synonyms

ERK 7, ERK 8, ERK7, ERK8, MAP kinase 15, MAPK 15, MAPK15

验证数据展示

  • WB analysis of mouse lung using 13452-1-AP

    WB analysis of mouse lung using 13452-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13452-1-AP (MAPK15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 13452-1-AP

    IP experiment of mouse lung using 13452-1-AP

    IP result of anti-MAPK15 (IP:13452-1-AP, 4ug; Detection:13452-1-AP 1:500) with mouse lung tissue lysate 3800ug.

  • IHC staining of human kidney using 13452-1-AP

    IHC staining of human kidney using 13452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13452-1-AP (MAPK15 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 13452-1-AP

    IHC staining of human kidney using 13452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13452-1-AP (MAPK15 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13452-1-AP targets MAPK15 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MAPK15 fusion protein Ag4255 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase 15
Synonyms ERK 7, ERK 8, ERK7, ERK8, MAP kinase 15, MAPK 15, MAPK15
Calculated Molecular Weight 544 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC028034
Gene Symbol MAPK15
Gene ID (NCBI) 225689
RRIDAB_2877950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD08
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAPK15 antibody 13452-1-APDownload protocol
IHC protocol for MAPK15 antibody 13452-1-APDownload protocol
IP protocol for MAPK15 antibody 13452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

Metastasis-associated gene MAPK15 promotes the migration and invasion of osteosarcoma cells via the c-Jun/MMPs pathway.

Authors - Zexin Su
View Article